| 角色 | 饰演者 | 角色介绍 |
| Sherlock Holmes | 巴兹尔·雷斯伯恩 | |
| Dr. Watson | 尼格尔·布鲁斯 | |
| Ann Brandon | 艾达·卢皮诺 | |
| Jerrold Hunter | 艾伦·马歇尔 | |
| Billy | Terry Kilburn | |
| Prof. Moriarty | George Zucco | |
| Sir Ronald Ramsgate | Henry Stephenson | |
| Inspector Bristol | E.E. Clive | |
| Bassick | Arthur Hohl | |
| Mrs. Jameson | May Beatty | |
| Lloyd Brandon | Peter Willes | |
| Mrs. Hudson | Mary Gordon | |
| Justice of the Court | 福尔摩斯·赫伯特 | |
| Mateo | George Regas | |
| Lady Conyngham | Mary Forbes | |
| Dawes | Frank Dawson | |
| Inquisitive Stranger | William Austin | |
| Tony Conyngham (as Anthony Kemble Cooper) | Anthony Kemble-Cooper | |
| Tompkins (uncredited) | Frank Baker | |
| Cockney (uncredited) | Frank Benson | |
| Pub Customer (uncredited) | Ted Billings | |
| Cragin (uncredited) | Harry Cording | |
| Tower Sentry (uncredited) | Robert Cory | |
| Clerk of the Court (uncredited) | Neil Fitzgerald | |
| Sergeant of the Guard (uncredited) | Denis Green | |
| Guard (uncredited) | Gordon Hart | |
| Bobby (uncredited) | Ivo Henderson | |
| Constable (uncredited) | Keith Hitchcock | |
| Bobby (uncredited) | Leyland Hodgson | |
| Bobby (uncredited) | Boyd Irwin | |
| Marine Sergeant (uncredited) | Charles Irwin |
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