| 角色 | 饰演者介绍 |
| Sherlock Holmes | 巴兹尔·雷斯伯恩 |
| Dr. Watson | 尼格尔·布鲁斯 |
| Ann Brandon | 艾达·卢皮诺 |
| Jerrold Hunter | 艾伦·马歇尔 |
| Billy | Terry Kilburn |
| Prof. Moriarty | George Zucco |
| Sir Ronald Ramsgate | Henry Stephenson |
| Inspector Bristol | E.E. Clive |
| Bassick | Arthur Hohl |
| Mrs. Jameson | May Beatty |
| Lloyd Brandon | Peter Willes |
| Mrs. Hudson | Mary Gordon |
| Justice of the Court | 福尔摩斯·赫伯特 |
| Mateo | George Regas |
| Lady Conyngham | Mary Forbes |
| Dawes | Frank Dawson |
| Inquisitive Stranger | William Austin |
| Tony Conyngham (as Anthony Kemble Cooper) | Anthony Kemble-Cooper |
| Tompkins (uncredited) | Frank Baker |
| Cockney (uncredited) | Frank Benson |
| Pub Customer (uncredited) | Ted Billings |
| Cragin (uncredited) | Harry Cording |
| Tower Sentry (uncredited) | Robert Cory |
| Clerk of the Court (uncredited) | Neil Fitzgerald |
| Sergeant of the Guard (uncredited) | Denis Green |
| Guard (uncredited) | Gordon Hart |
| Bobby (uncredited) | Ivo Henderson |
| Constable (uncredited) | Keith Hitchcock |
| Bobby (uncredited) | Leyland Hodgson |
| Bobby (uncredited) | Boyd Irwin |
| Marine Sergeant (uncredited) | Charles Irwin |
