| 角色 | 饰演者 | 角色介绍 |
| Osmosis Jones (voice) | 克里斯·洛克 | |
| Thrax (voice) | 劳伦斯·菲什伯恩 | |
| Drix (voice) | 大卫·海德·皮尔斯 | |
| Leah (voice) | Brandy Norwood | |
| Mayor Phlegmming (voice) | 威廉·夏特纳 | |
| Tom Colonic (voice) | 朗·霍华德 | |
| Kidney Rock (voice) | 摇滚小子 | |
| Kidney Rock (voice) | Kenny Olson | |
| Kidney Rock (voice) | Jason Krause | |
| Kidney Rock (voice) | Joe C. | |
| Kidney Rock (voice) | Stefanie Eulinberg | |
| Kidney Rock (voice) | Jimmie Bones | |
| Kidney Rock (voice) | Uncle Kracker | |
| Additional Character Voice (voice) | Jonathan Adams | |
| Additional Character Voice (voice) | 卡洛斯·阿拉兹拉奎 | |
| Additional Character Voice (voice) | Keith Anthony | |
| Additional Character Voice (voice) | Eddie Barth | |
| Additional Character Voice (voice) | Sally Ann Brooks | |
| Additional Character Voice (voice) | Rodger Bumpass | |
| Additional Character Voice (voice) | Michael Carven | |
| Additional Character Voice (voice) | Paul Christie | |
| Additional Character Voice (voice) | Janis Dardaris | |
| Additional Character Voice (voice) | Wendy Dillon | |
| Additional Character Voice (voice) | Antonio Fargas | |
| Additional Character Voice (voice) | Eddie Frierson |
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