| 角色 | 饰演者介绍 |
| Dr. Baron Victor Frankenstein | 彼得·库欣 |
| Anna Spengler | 维罗妮卡·卡尔森 |
| Professor Richter | 弗雷迪·琼斯 |
| Dr. Karl Holst | 西蒙·沃德 |
| Inspector Frisch | Thorley Walters |
| Ella Brandt | 玛克辛·奥德丽 |
| Dr. Frederick Brandt | George Pravda |
| Police Doctor | Geoffrey Bayldon |
| Mad Woman | Colette O'Neil |
| Third Houseguest (plumber) | Frank Middlemass |
| Fourth Houseguest (playing chess) | George Belbin |
| Second Houseguest (smoking pipe) | Norman Shelley |
| First Houseguest (reading newspaper) | Michael Gover |
| Asylum Principal | Peter Copley |
| Dr. Otto Heideke | Jim Collier |
| Police Sergeant | Allan Surtees |
| Sergeant Brenner | Windsor Davies |
| Burglar | Harold Goodwin |
| Policeman (uncredited) | Timothy Davies |
| Official (uncredited) | Robert Davis |
| Passer-By (uncredited) | Caron Gardner |
| Mortuary Attendant (uncredited) | Robert Gillespie |
| Warder (uncredited) | Michael Goldie |
| Water Board Workman (uncredited) | Edward Higgins |
| Christina Ella's Friend (uncredited) | Elizabeth Morgan |
| Lady In Garden (uncredited) | Daphne Oxenford |
| Anna's Neighbour (uncredited) | Dorothy Smith |
