| 角色 | 饰演者 | 角色介绍 |
| Dr. Temperance 'Bones' Brennan? (165 episodes 2005-2013) | 艾米丽·丹斯切尔 | |
| Special Agent Seeley Booth (165 episodes 2005-2013) | 大卫·伯伦纳兹 | |
| Angela Montenegro (165 episodes 2005-2013) | 米凯拉·考林 | |
| Dr. Jack Hodgins (165 episodes 2005-2013) | T·J·塞恩 | |
| Dr. Camille Saroyan (143 episodes 2006-2013) | 塔玛拉·泰勒 | |
| Dr. Lance Sweets (115 episodes 2007-2013) | 约翰·弗朗西斯·达利 | |
| Zack Addy? (61 episodes 2005-2010) | 艾瑞克·米勒甘 | |
| Caroline Julian (32 episodes 2006-2013) | 帕特里夏·贝尔彻 | |
| Dr. Daniel Goodman (22 episodes 2005-2006) | Jonathan Adams | |
| Wendell Bray (22 episodes 2008-2013) | Michael Terry | |
| Dr. Clark Edison (21 episodes 2007-2013) | 尤金尼·伯德 | |
| Max Keenan? (17 episodes 2006-2013) | 瑞安·奥尼尔 | |
| Daisy Wick (17 episodes 2008-2013) | 卡拉·盖洛 | |
| Christine Angela Booth? (14 episodes 2012-2013) | Susanne Allan Hartman | |
| Arastoo Vaziri (13 episodes 2009-2013) | Pej Vahdat | |
| Security Guard (13 episodes 2006-2011) | Joe Davis | |
| Christine Angela Booth? (13 episodes 2012-2013) | Ali Louise Hartman | |
| Colin Fisher (11 episodes 2008-2012) | 乔·大卫·摩尔  | |
| Vincent Nigel-Murray (11 episodes 2008-2011) | 瑞安·卡怀特 |
