| 角色 | 饰演者介绍 |
| Marcus Superbus | 弗雷德里克·马奇 |
| Empress Poppaea | 克劳黛·考尔白 |
| Emperor Nero | 查尔斯·劳顿 |
| Mercia | Elissa Landi |
| Tigellinus | Ian Keith |
| Titus | Arthur Hohl |
| Favius | Harry Beresford |
| Stephan | Tom Conlon |
| Glabrio | Ferdinand Gottschalk |
| Dacia | Vivian Tobin |
| Licinius | William V. Mong |
| Ancaria (as Joyzelle) | Joyzelle Joyner |
| Viturius | Richard Alexander |
| Strabo | Nat Pendleton |
| Servillius | Clarence Burton |
| Tybul | Harold Healy |
| Philodemus (as Robert Manning) | Robert Manning |
| Tyros | Charles Middleton |
| Bombadier - 1944 Re-Release Prologue (uncredited) | Joel Allen |
| Christian in Dungeon (uncredited) | 米沙·奥尔 |
| Bettor of 300 Silver (uncredited) | Lionel Belmore |
| Slave of Nero (uncredited) | True Boardman |
| Roman Woman (uncredited) | Marjorie Bonner |
| Mute Giant (uncredited) | Joe Bonomo |
| Spectator at Colosseum (uncredited) | Henry Brandon |
| Slave of Nero (uncredited) | George Bruggeman |
| (uncredited) | Horace B. Carpenter |
| Christian Martyr Saying "Victory" / Roman citizen outside of Colisseum / Gladiator saying 'We who are about to die.' (uncredited) | 约翰·卡拉丁 |
