| 角色 | 饰演者介绍 |
| Samanosuke Akechi (voice) | Robin Atkin Downes |
| Jacques Blanc (French) (voice) | 让·雷诺 |
| Jacques Blanc (English) (voice) | Paul Mercier |
| Ako (voice) | Amber Hood |
| Michelle Aubert (English) (voice) | Jennifer Hale |
| Henri Blanc (English) (voice) (as BJ Ward) | B.J. Ward |
| Nobunaga Oda (voice) | Richard Doyle |
| Ranmaru Mori (voice) | Joshua Keaton |
| Guildenstern (voice) | Neil Ross |
| Vega Donna (voice) | Vanessa Marshall |
| Tadakatsu Heihachiro Honda (Heihachi)/Gargant (voice) (as Dee Baker) | Dee Bradley Baker |
| Mitsuhide Akechi (voice) | Paul Eiding |
| Hideyoshi Hashiba (voice) | Armin Shimerman |
| Soldier (voice) | Daran Norris |
| Merchant (voice) | Bart Flynn |
| Worker (voice) | Ben Judd |
| Spirit of Onimusha (voice) | Dorian Harewood |
| Voice of Mother (voice) | 裘蒂·班森 |
| (voice) | Odile Cohen |
| (voice) (as Jacky Berger) | Jackie Berger |
| (voice) (as Maurice Decoster) | Maurice De Coster |
| (voice) | Roland Timsit |
| (voice) (as Jean Loup Horwitz) | Jean-Loup Horwitz |
| (voice) | Pierre Forest |
| (voice) (as Johana Menuteau) | Johanna Menuteau |
| (voice) | Nathalie Homs |
| (voice) | Soase Le Bras |
