| Frank James aka Ben Woodson | 亨利·方达
 | |
| Eleanor Stone | 吉恩·蒂尔尼
 | |
| Bob Ford | 约翰·卡拉丁
 | |
| Jesse Woodson James (archive footage) | 泰隆·鲍华
 | |
| Clem aka Tom Grayson | 杰基·库伯
 | |
| Major Rufus Cobb | 亨利·赫尔
 | |
| George Runyan | J. Edward Bromberg | |
| McCoy | Donald Meek
 | |
| Station agent - at Eldora | 艾迪·柯林斯
 | |
| Judge | George Barbier | |
| Prosecutor | Russell Hicks | |
| Pinky | Ernest Whitman | |
| Charlie Ford | Charles Tannen | |
| Randolph Stone | Lloyd Corrigan | |
| Preacher | Victor Kilian | |
| Colonel Jackson | Edward McWade | |
| Roy | George Chandler | |
| Bystander at wagon sale | Irving Bacon | |
| Sheriff | Frank Shannon | |
| Nellie Blane | Barbara Pepper | |
| Watchman | Louis Mason | |
| Mose (bellboy) (as Stymie Beard) | Matthew 'Stymie' Beard | |
| Actor playing Jesse James | William Pawley | |
| Pappy | Frank Sully | |
| Officer (as Davidson Clark) | Davison Clark | |
| Actor playing Frank James (uncredited) | Bob Battier | |
| Train engineer (uncredited) | Arthur S. Byron | |
| Deputy (uncredited) | Kernan Cripps | |
| Jury foreman (uncredited) | Edmund Elton | |
| Deputy (uncredited) | Budd Fine | |
| Mrs. Edna Stone (uncredited) | Almeda Fowler | |
| Court clerk (uncredited) | Sherry Hall | |
