| 角色 | 饰演者 | 角色介绍 |
| Jason Bourne | 马特·达蒙 | |
| Nicky | 朱丽娅·斯蒂尔斯 | |
| Ross | 帕迪·康斯戴恩 | |
| Paz | 埃德加·拉米雷兹 | |
| Pamela Landy | 琼·艾伦 | |
| Jimmy | 丹·弗雷登堡 | |
| Noah Vosen | 大卫·斯特雷泽恩 | |
| Ward Abbott | 布莱恩·考克斯 | |
| John St. Jacques | 丹尼尔·布鲁赫 | |
| Desh | 乔伊·安沙 | |
| CRI Operative | Trevor St. John | |
| Tom Cronin | Tom Gallop | |
| Neal Daniels | Colin Stinton | |
| Lucy | Lucy Liemann | |
| Technician | Bryan Reents | |
| Technician | John Roberson | |
| Technician | Russ Huards | |
| Betancourt | 马克·贝兹利 | |
| Chamberlain | Sinead O'Keeffe | |
| Russian Policeman #1 | Branko Tomovic | |
| Russian Policeman #2 | Laurence Possa | |
| Tactical Team Agent | Albert Jones | |
| Vosen's Driver | Jeffrey Lee Gibson | |
| Morgue Attendant | Uriel Emil Pollack | |
| NYPD Officer #1 | Omar Hernández | |
| NYPD Officer #2 | William H. Burns | |
| CRI Agent | Michael Wildman | |
| Hoody | Kai Martin | |
| Technician | Arkie Reece | |
| Agent Hammond | Chucky Venice | |
| Commuter (uncredited) | Chris Mansfield | |
| Marie Kreutz (archive footage) (uncredited) | 弗兰卡·波坦特 | |
| Security Guard (uncredited) | Walter A. Saunders III | |
| News Agent (uncredited) | John Snowden | |
| Policeman (uncredited) | John Warman | |
| Nabile (uncredited) | Ben Youcef | |
| Hoody | Kai Martin |
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