| 角色 | 饰演者介绍 |
| Mario Mario | 鲍勃·霍斯金斯 |
| Luigi Mario | 约翰·雷吉扎莫  |
| King Koopa | 丹尼斯·霍珀 |
| Princess Daisy | 萨曼莎·玛西丝 |
| Iggy | 费舍·史蒂芬斯 |
| Spike | Richard Edson |
| Lena | 菲奥娜·肖 |
| Daniella | Dana Kaminski |
| Toad | Mojo Nixon |
| Scapelli | Gianni Russo |
| Bertha | Francesca Roberts |
| The King | 兰斯·亨利克森 |
| Old Lady | Sylvia Harman |
| Angelica | Desiree Marie Velez |
| Brooklyn Girl | 安德瑞·鲍威尔 |
| Brooklyn Girl | Heather Pendergast |
| Brooklyn Girl | Melanie Salvatore |
| Goomba Toad | John Fifer |
| Sergeant Simon | Don Lake |
| Hatcheck Girl | Terry Finn |
| Goomba | Thomas Merdis |
| Goomba | Michael Harding |
| Goomba | Michael Lynch |
| Goomba | Scott Mactavish |
| Goomba | Wallace Merck |
| Nun | Mona B. Fierro |
| Nun | Karen Brigman |
| Nun | Christi Work |
| Nun | Joy Rees |
| Nun | Lucy Alpaugh |
| TV Announcer | Robert D. Raiford |
| Reporter #1 | 哈里·墨菲 |
| Reporter #2 | Patt Noday |
| Scapelli Bodyguard | Robert Lee Edwards |
| Scapelli Bodyguard | Ronald Lou Edwards |
| Pizza Deliveryman | Matthew Zachary Hopkins |
| Egan (as Robert Faulkner Priester) | Robert Priester |
| James | Preston Lane |
| Japanese Businessman #1 | Jim Asaki |
| Japanese Businessman #2 | Matt Nikko |
| Devo Controller | Kevin West |
