| 角色 | 饰演者 | 角色介绍 |
| Batman / Bruce Wayne (voice) | 布鲁斯·格林伍德 | |
| Red Hood / Jason Todd (voice) | 简森·阿克斯 | |
| The Joker / Additional Voices (voice) (as John DiMaggio) | 约翰·迪·玛吉欧 | |
| Nightwing (voice) | 尼尔·帕特里克·哈里斯  | |
| Ra's al Ghul (voice) | 詹森·艾萨克 | |
| Black Mask (voice) | 韦德·威廉斯 | |
| Chi Chi / Thug (voice) | 卡洛斯·阿拉兹拉奎 | |
| Leon / Thug (voice) | Robert Clotworthy | |
| Bobo / Commissioner Gordon (voice) | 盖瑞·科尔 | |
| Ra's Assistant (voice) | Brian George | |
| Ms. Li / Talia al Ghul (voice) | 胡凯莉 | |
| Rick / Additional Voices (voice) | 菲尔·拉马 | |
| Young Robin / Young Jason Todd (voice) | Alexander Martella | |
| Robin / Jason Todd - Teen (voice) | 文森特·马特拉 | |
| Alfred Pennyworth (voice) | 吉姆·皮多克 | |
| Tyler / Additional Voices (voice) | Kevin Michael Richardson | |
| Reporter #1 / Additional Voices (voice) | Andrea Romano | |
| Freddie / Drug Dealer (voice) | Dwight Schultz | |
| Mercenary #1 / Amazo (voice) | 弗莱德·塔他斯西奥 | |
| Reporter #2 / Female Assassin (voice) | Keri Tombazian | |
| The Riddler / Additional Voices (voice) (as Bruce Timm) | Bruce W. Timm |
