| 角色 | 饰演者介绍 |
| 谢洛克·福尔摩斯 Sherlock Holmes | 尼科尔·威廉森 |
| 华生医生 Dr. John H. Watson/Narrator | 罗伯特·杜瓦尔 |
| 弗洛伊德 Dr. Sigmund Freud | 艾伦·阿金 |
| Lola Deveraux | 瓦妮莎·雷德格瑞夫 |
| Professor James Moriarty | 劳伦斯·奥利弗 |
| Baron von Leinsdorf | 杰瑞米·坎普 |
| Lowenstein | 乔尔·格雷 |
| Mary Morstan Watson | 萨曼莎·艾加 |
| Madame | Régine |
| Mycroft Holmes | 查尔斯·格雷 |
| Mrs. Freud | Georgia Brown |
| Freda | Anna Quayle |
| Mrs. Holmes | Jill Townsend |
| Berger | John Bird |
| Mrs. Hudson | Alison Leggatt |
| Marker | Frederick Jaeger |
| James The Butler at Mycroft's Club | Erik Chitty |
| Dr. Schultz | Jack May |
| The Pasha | Gertan Klauber |
| Squire Holmes | Leon Greene |
| Young Holmes | Michael Blagdon |
| Young Freud | Ashley House |
| Nun | Sheila Shand Gibbs |
| Station Master | Erich Padalewski |
| Train Engineer | John Hill |
| (uncredited) | Drewe Henley |
